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[CDR3序列.xlsx](https://github.com/liulab-dfci/TRUST4/files/13934536/CDR3.xlsx)
Thanks to the developers of the tool for facilitating us to analyze the immune repertoire data. I used the trust-smarts…
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Dear DALI team,
Few questions.
1) Can you please provide a link or code on how you built the seuratObj.rds object? It will help us use the app if we can reproduce your your .rds object as well a…
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### Description of feature
This issue is to provide an overview of different tools and related sub-issues related to supporting multiple modalities:
- CITE-seq (=RNA+protein) (https://github.co…
grst updated
4 months ago
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Hi,
Is there way to extract reads that were successfully assembled by vdjer from the original bam file?
I mean the following. I supposed that `vdjer.sam` contains reads that were assembled by vdjer…
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Hello, I am a TRUST4 beginner, so the questions may be quite basic. I hope you can patiently answer them.
In my studies, I encountered some situations where when I read a directly downloaded Bam file…
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Thank you for developing this tool !
I work on a bulk RNA-Seq Illumina sequencing result (BCR sequences have been caught using a C primer and a 5‘RACE protocol). We have introduced UMI in these se…
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Bowtie2 seems to return very low VDJ alignment rates for each of my B cells and virtually no VDJ sequences were able to be reconstructed (BCR_summary.txt screenshot linked below).
Because bracer w…
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- [ ] Antigen affinity score
* Incorporates AVID-seq counts and total receptor counts (CITE-seq/GEX counts)
- [x] Add VDJ data from cellranger to Seurat object
- [x] Receptor diversity
* inver…
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**Description**
As a data wrangler wrangling a project with 10X 5' V(d)J data I need to determine the correct way of defining the experiment in our spreadsheet and determine whether any a…
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Hello, I am currently trying to analyse the clonotype information from a pooled BCR sample. Here the DNA barcodes were used to differentiate the sample of origins from a single captured 5' VDJ scRNA-s…