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Hello all,
Thank you so much for creating and sharing such a great package.
Would it be possible to use TiSA for proteomics analysis? Do I need to follow a binomial distribution to use your packag…
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### Rebuild the software suite
The software and the web wrapper require some more work to make them more useful for others, and we decided to try to allow this software package to handle the whole pr…
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#### Q: Should housekeeping genes be configurable?
I'm not 100% sure anymore, but I think I remember that you mentioned that the housekeeping genes should be configurable. If so, this code should b…
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The working directory is currently hard-coded:
https://github.com/bjbvoesenek/qPCR-analysis/blob/2b49bf13fba765547933ca3be6fe03b8499e2478/qpcr_analysis.py#L12-L15
This makes it currently impossibl…
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Although the web wrapper will make sure there are three arguments, the code does not check for it. It's common practice to handle such cases nicely.
Instead of the current output:
```
Traceback (…
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The script fails hard when the given housekeeping genes are not found. This includes when they are given simply in the wrong case, e.g., `gapdh` instead of `GAPDH`, or `bactin` instead of `Bactin`.
…
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Hi!
I have two BAM files where the contents are identical except for the headers of the reads. I ran TEcounts for both and I am getting tiny differences that I cannot explain... For example, gene_A…
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https://mp.weixin.qq.com/s/ejNXRnr3GretIVcKlK2nDQ
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Hello,
I am having some trouble with our first NanoSeq results, but I am not sure whether this is a wetlab or bioinformatics problem (though I suspect the former). I processed the data as far neces…
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Hey,
how to read excel tables that don't have their headers on the first row? Many scientific applications export their data with printing device information into the first rows and then following wi…