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I am running dorado/dorado-server on a SLURM cluster, and there is a very high performance partition that I can access but it can kick me off spontaneously if the "owner" of a machine starts up a job,…
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# Issue Report
## Please describe the issue:
I am trying to run dorado on a .pod5 file. However, when I do so, I get an error:
[2024-04-19 23:00:41.196] [error] finalise() not called on a Hts…
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With current version of MinKNOW/dorado_basecall_server on a fast SSD with a `Nvidia RTX A6000 (48G)` how fast is "adaptive sampling"? Translocation speed seems to be around 350 bases/sec.
Or how ma…
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Hello, I have been using dorado 0.4.1 to re-basecall and demux some R9.4.1 reads. using the HAC model, I get mostly unclassified reads (>90% unclassified), when previously there was less than 5% uncla…
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Hi,
I was playing around with different modification models on a DNA dataset.
I tested both 5mCG_5hmCG@V3 and 5mC_5hmC@V1 models (with sup@v4.2.0 as basecalling model).
I wonder which are the di…
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Hello, I'm a student currently working on my master's project. Given that our lab is taking its first steps into nanopore sequencing, we're navigating somewhat blindly and lack sufficient resources fo…
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When basecalling modified bases with `dorado` v0.5.0, using the modification models `5mC_5hmC` and `5mCG_5hmCG` stall basecalling randomly, i.e. sometimes `dorado` finishes basecalling, but mostly it …
janpb updated
8 months ago
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Hi Hiruna,
This looks like a really great tool, and I wanted to thank you for your detailed documentation of distinction between generating nucleotide-anchored signal with f5c and what you get from…
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I'm struggling with FAST5 playback as described in the README with MinKNOW 20.06.5. I get zero pores and no reads (like #65). However, I'm trying to replay the bulk fast5 linked in the README.
Of …
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Hi dorado Team,
I have some issues with old data converted to pod5. I had a lot of data set which I currently coverting to pod5 for re-basecalling with dorado. However, for some of the sample, althou…