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https://mp.weixin.qq.com/s/TgKx0XDB62nvbtG6hwU0xg
ixxmu updated
2 years ago
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Hi,
Thank you for developing this great tool.
Do you recommend log2TPM or TPM for bulk RNA seq data? I think this results in different phenotype association.
Thanks!
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https://mp.weixin.qq.com/s/hhhQJZzfe1irDzcE29P9Zg
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https://mp.weixin.qq.com/s/QcoW8ekIG7Pr6KMf74Jp3A
ixxmu updated
2 years ago
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For bulk-RNA analysis I propose the following approach.
If de novo editing sites want to be called:
1) Run pileup on a bam file (`get_pileup()`) to detect variants above certain read depth thresh…
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https://mp.weixin.qq.com/s/3lzRv7BrXgXd6GB_UI0axw
ixxmu updated
2 years ago
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Hi bro. Do I need to construct a reference genome for Dynascope's Multi_? What species does the test data correspond to？
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### Context
Came up in my discussion with @cgreene yesterday.
### Problem or idea
There is metadata available on MetaSRA for some experiments on refine.bio. We can use that instead of the refin…
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您好，我第一次接触DNBelab C4平台的单细胞转录组的数据，我通过：
`$PISA parse2 -x C4 -t 8 FASTQ_R1 FASTQ_R2 -1 FASTQ_OUT`
将FASTQ转换成FASTQ+。
但通过质控或者有exacly matched barcode的reads普遍比总数低一个数量级，请问这种情况正常吗？
还是说我的文库结构和PISA预设的C4文库结构不一…
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https://mp.weixin.qq.com/s/nAy7FGhNqt2NywDzNO2bEQ
ixxmu updated
2 years ago