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Hello, I have a question about the data used for the fungal ITS DADA2 tutorial (https://benjjneb.github.io/dada2/ITS_workflow.html)
I understand you received the mock community from Bakker et al 20…
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**User story**
GPL-357 As Analysis lead (David Jackson) I would like a new sequencing type of 'Amplicon' to support analysis from the MLWH
**Who the primary contacts are for this story**
David Ja…
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Hi there, I was evaluating whether MiXCR could be used to analyze [Clontech RACE TCR data](https://www.takarabio.com/assets/documents/User%20Manual/SMARTer%20Human%20TCR%20ab%20Profiling%20Kit%20User%…
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Background:
We have sequenced 10 samples of metagenomics data using illumina ampliseq. Its is 16S metagenomics panel. The data set consist of 301bp read length, paired end reads. The quality of th…
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How do we begin annotating the microbial taxonomy with morphological and physiological properties? For example:
* [obligate aerobes](https://en.wikipedia.org/wiki/Obligate_aerobe)
* [facultative ana…
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Hello,
I am using dada2 for analyzing 16S amplicon data. Raw sequences were obtained by sequencing the V4-V5 region of the 16S using primer 515F/926R on a 2X300 bp Miseq platform. I didn't encounter …
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Hi Ben,
I know this issue has been posted many times. I tried to follow older posts but could not resolve my issue.
I am running DADA2 pipeline for my v3-v4 amplicon analysis with F primer: 343F (5…
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I want to use bcbio to process TruSeq Custom Amplicon (TSCA) data. However, I notice the performance of bcbio's variant2 workflow is not great (comparison to MiSeq reporter workflow below).
I comp…
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Hi
I have three questions about features I cannot see in the documentation but wonder if they exist:
1) When a coding sequence is provided the Frameshift_analysis.txt gives a summary of the numb…
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Let me know if this is a question better asked in another forum. I'm using mothur to do some QC on data from a highly multiplexed amplicon sequencing panel (~135 primer pairs x 48 samples). I'm having…