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Add "Highly targeted resequencing", "Targeted next-generation sequencing panels", "Amplicon panels", "Panels", "Amplicon-based sequencing" as narrowSynonym of "Sequencing" ... more likely making "Res…
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I recently read your paper titled “High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution. Nucleic Acids Research, 2019.” I am currently applying your d…
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when I use vsearch for amplicon sequencing data analysis, I have installes vsearch
when I use it, something wrong happended:
`ubuntu@ip-172-31-14-112:~/LC$ time vsearch ‐‐usearch_global seq18s-A…
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Hi,
I have read the FAQ and saw this question is addressed but I do not know how to proceed.
I have 742 paired end samples from Ilumnina HiSeq runs that I demultiplexed. Barcodes and primers (51…
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Hi all, I am trying to analyse with DADA2 some PE reads downloaded from SRA but when I merge them after Dada2 pipeline I get very low output (range 0-10). Is there an additional step that I am missing…
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I started working on this
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Hi I am new to DADA2 and am trying to learn how to convert sequencing data into OTU tables. I have a WGS read level data where I have fastq paired end data files for each sample. Can I process this d…
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Hi team,
I'm looking to call the variants from multiple amplicons in single sample.
Clearly a sample contains **10genes** to call variants using nanopolish variants. And I don't want to mention the…
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I am trying to analyze a data set that only the R1 reads are available, although it was sequenced as paired-end. Specifically (besides not adding any fnFrs, etc. with anything R in it) what do I need …
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Hello! I was wondering if you could separate each barcoded cells reads into a new fastq file, 1000 to 10,000 fastq files per 10x genomics experiment. Do you think SONAR would be able to process them…