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Hi,
Thanks for this great tool and the great support you guys have provided here!
I had previously posted about running TRUST4 on multiplexed 10X 5' human PBMC scRNA-seq samples (https://github.…
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Hi Zijie,
Thanks for this useful package! I am wondering will the pipeline support 10X bam files in the future? If not, could I just modify the STAR arguments to align with the needs of 10X bam fil…
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Hi,
Can you please give a suggestion on how to use SEACR with experiments that have spike-in and replicates. What do you recommend as far as normalization with spike-in and how to handle replicates?
…
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When I run giremi.pl, I will get an error in the *.Rout file. The error message is:
Error in data.frame(..., check.names = FALSE) :
参数值意味着不同的行数: 0, 1
Calls: cbind -> cbind -> data.frame
And here is…
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Dear Haibao,
I try to compute dotplots with new assemblies anchored by allmaps of two closely related species.
Both assembly annotation show about 35'000 gene models. But when calculating ortholog…
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Hi all,
I am using JAFFA-2.4 to discover the BCL2 fusions in FFPE target RNA sequencing data.
While this sample had been verified positive using FISH, with partener gene IGHJ, which had been ver…
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As we are evaluating integration methods and testing subsets of datasets, it would be helpful to see the exact breakdown of ScPCA projects and how much of each disease, 10X platform, etc. are included…
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Hello,
Using trimming methods M1, my trimmed reads are too short to overlap and an erroneous contig is created (see below numerous degeneracies). Relaxing M1TrimmingCutoff to 0.0002, the merge is p…
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Hello,
I am not sure I understand the meaning of the -c flag "-c Smaller size allowed for an intron created for genes. Default: 16. We recommend to use the reads length". Why do you recommend the …
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Dear Prof
I trust you are keeping well
I want to know, that is it necessary to have phylogenetic tree for this model, or we can use this with out tree
kindly please let me know
Thanks and Regards