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Dear professors:
After compressing the raw fast5 file by ont_fast5_api. I try to use the vbz-compressed fast5 to call mathylation by Nanopolish software. The error occurs likes below:
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Hi,
Thanks for your work on this program. The method seems really elegant and we've had some really exciting results from it so far.
Here is a brief outline of our preliminary analysis:
- We…
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You may want to also add genetic demultiplexing as a complementary approach (or a ticket to have it as a possibility) if folks multiplexed samples. It may also reveal if there are free-floating nuclei…
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Hello,
This might be an obivous question, i guess your terminology in the description is confusing me. When you mean Raw HTO counts, do you mean non normalised HTO UMI count matrix, or do you mean th…
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I have been getting this error
```
Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:RNA_FUSIONS_JAFFAL:UNTAR (null)'
Caused by:
Not a valid path value type: org.codehaus.groovy.runtime.NullObj…
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I have a very complex query (about 200 terms) with many Booleans operations, but I have noticed that there is a limit to how long my query can be before the query stops being validated. I have verifie…
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using latest ethers 5.0.30
test code:
```
const url = '/geth.ipc'
const ethers = require('ethers')
const provider = new ethers.providers.IpcProvider(url);
provider.on("pending", console.log)
``…
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Hi,
Is there a way to make demultiplexing procedure any faster? Even with 32 cpus and 200g mem - it takes 2 days to finish for a 30g fastq files. If you could please implement a faster version som…
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Hi -
I'm struggling to get Circuitseq running on my data. I've successfully run it on the example data, but when I try to run on my own data I get the same behavior as reported in #12 - Nextflow ru…
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woltka classify for align/bowtie2/S01.sam.xz
```
woltka classify --to-tsv -f sam -i S01.sam --digits 4 -o sam.txt
```
- logs
Input alignment file: S01.sam.
Demultiplexi…