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Hello,
After running conga, I have the following conga cluster:
![image](https://user-images.githubusercontent.com/10944134/139119742-d8c04009-d2fd-41df-b4e3-38def481bb15.png)
When I look at t…
s2hui updated
2 years ago
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Hello @mckellardw!
Thanks for sharing the large integrated dataset of muscle scRNA-seq.
It is a very valuable referece for further analysis.
I downloaded the raw object of seruat. And I found the m…
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Hi, my name is Rebecca Combs. I am a student at Winsor High School in Boston. I'm fascinated by scRNA-seq data analysis. I have run into a glitch. I'm unable to complete the [Nestrorowa et al. 2016 pa…
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Hi,
I used Seurat to do clustering, then singleR to do cell annotation. In this annotation, I got multiple cell types such as B cells and T cells and Epithelial cells. I'm only interested in Epithe…
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Currently, we are analyzing our scRNA-seq data obtained from the mice through Compass platform. I want to ask if we can use “extdata/RECON2/reaction_metadata.csv”(suitable for human in your analysis) …
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Let's discuss how we incorporate spatial scRNA-seq into our workflow. We'd need to add spatial plots, and spatially variable features, but for the most, it is the exact same flow of analysis.
http…
ktrns updated
2 years ago
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So I am analyzing a scRNA seq from homogenized tissue from different samples, I've two questions here,
1 - Do I integrate by sample or by sequencing run? I have no information whether the samples we…
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https://mp.weixin.qq.com/s/dYK_J5y6lXLpxE3QUNaP7g
ixxmu updated
2 years ago
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Dear Signac team,
I am encountering issues with the macs2 function that I am unable to solve and I hope that you may be able to provide me with some insights. I saw the other peak calling issues #4…
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## Describe the bug
I ran into this bug when trying to pack up the whole project and test reproducibility. I notice that the pipeline cannot reproduce even with set.seed in code. One example is "addD…