-
```
I'm using the fastq-mcf (1.04.662) in my pipeline to filter adaptors and trim
reads by quality.
I have used it for a methylation sequencing run and I didn't change the skew
parameter, I left th…
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I used porechop to process the SRR13602435.fastq file, but there were no changes in the resulting fastq file.
Then, I aligned the SRR13602435.fastq to mm10.combined.fa using minimap2 V2.17. After r…
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Dear Sir/Madam,
Hope you are well.
I download the original bam file and did a bamtofastq convert. So I found that SRR7092170 has a bam. file (link: https://trace.ncbi.nlm.nih.gov/Traces/?view=r…
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Hi,
I have a question regarding how you use the FASTQ description field to calculate the hash. The question arises because I've been doing some tests and I only manage to get the same md5 using the `…
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Hi, I am trying to calculate the kmer profile of this 5.0 Gb genome. Here's the command:
kat comp -t 32 -m 17 -o genome1VSgenome2 -h 'fastq1_R1.fastq.gz fastq1_R.fastq.gz fastq2_R1.fastq.gz fastq2_…
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```
What steps will reproduce the problem?
1.
2.
3.
What is the expected output? What do you see instead?
I have multiple gzipped fastq file:
I run STAR with --readFilesIn set to be:
--readFilesI…
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Hi, ursky！
Because I wish to publish result recently when I used the quant_bins module just to get metabat2 bins' abundance heatmap, however, metaWRAP presented this error.
```
Exception : [std::ba…
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### My command is:
simulator.py genome --ref_g $file \
--model_prefix ${Model_Output} \
--output ${output_dir1} \
--number 300 \
…
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Hi,
During the simulation of ultra-long ONT reads, an error was encountered: "fastq is too long. Max acceptable length is 1000000."
Will the program be updated?
Best.
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Hi @leepc12 , first, thank you for the great pipeline here 🙏
## **Describe the bug**
The failure is partially due to my input fastqs having identical file name and differs as different file on pa…