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https://mp.weixin.qq.com/s/Rv0QDcs5j4ssn5q3w5Cz7Q
ixxmu updated
9 months ago
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Hi everybody,
we have got 10 bases UMI in the header of fastqfiles. It is codified by RX tag.
We are using GATK to find somatic variants with low VAF.
Unfortunately we found that after fastqtosam…
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Re-write script to handle NovaSeq X (3000 no longer planned for ESP LIMS).
Need to also update / replace sequencing ESP nodes downstream.
Filename should be based on Flowcell ID in LIMS worksheet.
…
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Hello,
Why do the total values in `completeness.stats` and `qv` files differ so much? What do they represent and how they relate to each other? I run `merquryfk` with a single genome assembled usi…
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I am wondering if the outputted plain-text file also contains PS tags in addition to HP tags. As you suggested, both pieces of information are important to determine haplotypes. Thanks.
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I can think of cases where keeping the text before the first underscore could lead to naming collisions between different samples (e.g., if someone had samples like `P_putida`, `P_aerugi…
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Hello! I am interested in calculating aFC for genes with multiple causal variants and was pointed to this tool by Stephane Castel.
This seems like a really great extension of the aFC tool! I was cu…
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Index fields are hidden in the 'SEQ Lab: Create Illumina Library Pools' but are needed in the Illumina Sequencing Setup protocols, so not sure what's going on here. Will need to reach out to @mcordes-…
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### Description of the bug
Hello,
I am testing TaxTriage and running into an issue that I am having trouble resolving.
I have two samples, below is my sample sheet:
```
sample,single_end,fast…
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### Description of bug
Hi Phil,
I am having some issues with multiqc when running within viralrecon. The issue seems to happen when multiqc is looking at files `*.variant_counts_mqc.tsv` which …