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Hi,
I am trying to demultiplex my CITEseq data by MULTIseqDemux().
My data set includes Totalseq Ab (130 antibodies), Hashtag Ab (2 antibodies), and RNA information.
I set default assay with "HT…
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I started using `fqtk` yesterday, and it's really easy to install, plus nice and quick to use with a clear help page :+1:
Right now, we can specify the [read structure](https://github.com/fulcrumg…
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Hi @yuukiiwa, I really want to run this workflow but having some issues here. Can you please take a look?
### Description of the bug
Module compilation error
- file : /my/path/to/.nextflow/ass…
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Hi!
I managed to run the latest version of toulligQC (2.3) with default guppy basecalling:
```
toulligqc \
--report-name "$run_id" \
--telemetry-source ./fastq_hac_400/sequencing_telemetry.js \…
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hey!
I'm trying to use Assign_Indiv_by_Geno.R function of Demuxafy.sif from data of freedemuxlet code. It seems the problem is with the FORMAT, but I don't undestand very well...
apptainer exec …
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Dear professors:
After compressing the raw fast5 file by ont_fast5_api. I try to use the vbz-compressed fast5 to call mathylation by Nanopolish software. The error occurs likes below:
---------…
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You may want to also add genetic demultiplexing as a complementary approach (or a ticket to have it as a possibility) if folks multiplexed samples. It may also reveal if there are free-floating nuclei…
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I have recently updated my conda, in which I had Ipyrad running since 2021. Then, ipyrad started to give errors in step 2, appararently some incompatibility with cutadapt. The error occurs for each sa…
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I have been getting this error
```
Error executing process > 'NFCORE_NANOSEQ:NANOSEQ:RNA_FUSIONS_JAFFAL:UNTAR (null)'
Caused by:
Not a valid path value type: org.codehaus.groovy.runtime.NullObj…
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Hello,
This might be an obivous question, i guess your terminology in the description is confusing me. When you mean Raw HTO counts, do you mean non normalised HTO UMI count matrix, or do you mean th…