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@alexdobin
Hey Alex,
I'm using the STAR-aligner (Version 2.7.9a) to map human RNA-samples to a reference genome and to detect fusions for a self build Pipeline. The indexing of my reference gen…
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Hi,
I try to find the TSS with READemption in my RNA-seq, and there is an error. I have install the segemehl 0.2.0 on the PC and add it to the environment path. So could you please help me? Thanks. …
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## Check Documentation
I have checked the following places for your error:
- [X] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
- [X] [nf-core/sarek pipeline docum…
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**Software versions**
Specify what versions of the following you are using:
- `HTSeq`HTSeq 2.0.1
- `Python`Python 3.9.7
- operating system Linux
- (if applicable) `ST…
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Hello,
I was wondering if it is possible to use sideRETRO on non-human samples. When I run sideRetro on my samples, I get the following warning: "WARN merge_call.c:239: No cluster has been found!"…
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I am trying to get the number of reads mapped to regions in a BED file.
The BAM file is sorted using:
`samtools sort KAPA.bam KAPA.sorted`
The BED file is sorted using:
`sort -k1,1 -k2,2 regions.b…
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Hi,
I am trying to build genome for Mus musculs with getGenomeAndAnnotation function:
```
annotation
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### Description of the bug
When providing a gz compressed transcript file with `--transcript_fasta ` the pipeline errors with
```
[- ] process > NFCORE_RNASEQ:RNASEQ:PREPARE_GENOME:GUNZIP_G…
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### Description of the bug
I a running a rnaseq data on nfcore rnaseq for DEG analyses on uppmax. But I have problem here, its cancelled every time in the following step. why? how to overcome this ?
…
abu85 updated
2 years ago
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Hi I am trying to use nf-LO pipeline and I am running into some errors. On my server I had specified the nflo pipeline to use 8 cpus and 32gb of RAM but it fails with the error " **Process requiremen…