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I am trying to run the 1st steps of filtering the data using Seurat. I am using the freely available single-cell dataset from Zeishal et al. with the columns as cell i.ds and rows as gene names and th…
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First, I would like to say that I am so very happy to see a standardized scRNAseq visualization package such as this, which has made my work so much easier. I noticed when using the ```do_LigandRece…
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@anjaf @sfexova @ngeorgeebi @pnejad
Cc @zoependlington @dosumis
There are 2 terms in EFO
[EFO:0007832 'single cell sequencing'](https://www.ebi.ac.uk/ols/ontologies/efo/terms?iri=http%3A%2F%2F…
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```
> #Compute the relative expression of each gene per cell
> rel_expression
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### Description of the bug
when running the nf-core/scrnaseq full test on a Nextflow Tower Forge deployment with Wave containers, I get the following error:
```
Error executing process > 'NFCORE_SC…
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Hi all,
I am a new learner in using Seurat package to run my scRNAseq analysis. My problem is I have to analyse T cells (CD4 and CD8 T cells) and rare population of T cell subsets. I spiked my rar…
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Hi everyone, I'm new to single cell so I apologize if any of this is due to my experience. I had previously used a prior version of harmony without much trouble (unfortunately I don't remember which v…
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Hi, I have 10X genomics 3' scRNAseq data that I've processed through cellranger. I have the possorted bam output file and I am trying to run regtools to obtain the splice junctions bed file. Since cel…
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# 生信爱好者周刊(第 96 期):
这里记录每周值得分享的生信相关内容,周日发布。
本杂志开源(GitHub: [openbiox/weekly](https://github.com/openbiox/weekly)),欢迎提交 issue,投稿或推荐生信相关内容。
[「生信周刊讨论区」](https://github.com/openbiox/weekly/discussi…
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As we head into May 2023's intro scRNAseq workshop, we're rearranging some topics. We'll be moving the pathway analysis day to advanced, and moving the cell typing to intro.
For this issue, address…