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Hi there,
I recently worked with a dataset with 480 samples and it ran for hours and did not produce an output. So I reran it with just 10 and debugging commands before every command, and it has b…
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Hi Felix,
I wonder if there is a way to have the cytosine_report file from the coverage2cytosine function but only with the relevant information.
I have amplicon sequencing data, therefore I only h…
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kubu4 updated
5 years ago
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For example, polyN have trouble to sanger sequencing, therefore it is better not to place primer pairs around it.
The tag `SEQUENCE_EXCLUDED_REGION` is not suitable, as it tells primer3 to exclude …
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Thanks for publishing this tool!
I'm testing InSilicoSeq for simulating targeted sequencing of short amplicons. I often run into `string index out of range` error messages when I try to simulate re…
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Hi developer, I saw several questions here about PCR amplicon but still confused with my results so need your help badly.
My data is generated by ONT nanopore sequencing. I sequenced 5 different ampl…
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Dear Luca,
I am encountering the below error when running the command:
CRISPResso
-r1 /work/rpapa/sbelleghem/mutant_miSeq/fastq_trimmed/TL12_R1_paired.fastq.gz
-r2 /work/rpapa/sbelleghem/mut…
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Hello,
at first, I would like to thank you for dada2. I try to analyze microbiome of soil samples using this software and I have found some problems. Therefore, I would like to ask you for some advi…
JoBrz updated
4 years ago
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I am analyzing a functional gene amplicon sequence dataset, and I am wondering if you could answer some of my questions below?
- I'm curious whether DADA2 is an appropriate pipeline to process func…
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hi, thanks for your powerful software.
you give a detail description of how to use cnvkit for hybrid capture and Targeted Amplicon Sequencing (TAS), it has a good relationship with the THetA, I mean …