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- [x] passing custom cost matrices
- [ ] `LineageProblem`: passing barcodes
- [ ] `problem_stage`
- [ ] `plot_cell_transition`
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For your function for producing reverse complements you have:
"for each sequence convert to reverse complement."
It'd be good to go into a little bit more detail about the way this is done - switch …
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Hi- I am attempting to repeat what worked previously on a smaller data set where I added in a column to re-assign cell IDs to a specific sample. I have several longitudinal samples which I ran using c…
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Hi all,
would it be possible to add a zoom option/parameter, so barcodes from a longer distance would get recognized?
Best regards
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demultiplexer failed silently at execution time when no barcodes were supplied.
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I am working with some data with many `.h5ad` files stored in the same directory. Whenever I create a new `SpatialData` object I specify the path to the correct `AnnData` counts file.
I noticed that…
dawe updated
6 months ago
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In ASP.Net, when report is exported to PDF no barcodes shows up in PDF
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Hello,
After running your software, I noticed that some beads were classified as originating from the same cell. Could you please explain how this determination is made? Specifically, I'm curious a…
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I have a paired-end single-cell RNA-seq dataset. R1 consists of all the reads, and R2 of the barcodes necessary to identify which cell a read belongs to. If I now only trim R1 to keep high-quality rea…
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- Cutadapt and Python version used: 4.8
- The method you used to install the tool (conda or pip, for example): Used mamba
- The command-line parameters you used for demultiplexing paired-end reads w…