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Dear developer(s),
I'm facing an issue while trying to run the barcode tool in order to extract cell barcodes and UMIs from reads as part of some ongoing tests.
Essentially, the apparent usage o…
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I ran this command and it gave me CRISPRessoPooled -r1 TREATED_R1.fastq.gz -r2 TREATED_R2.fastq.gz -f crispressoexp_config.txt -x Genomes/hg38/genome --name AMPLICONS_AND_GENOME --gene_annotations ge…
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- [x] Consider changing the name of SpotID here to a more spcific terms such as RNASpotID (not to confuse with DNASpotID)
**The following items are missing and I think they would be useful to have:…
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https://mp.weixin.qq.com/s/Q8_6GJLb02kW_EZ_fn8jAQ
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Hi,
Would it be possible for demuxmix to model the HTO data together with VDJ data, rather than GEX data, where
for VDJ data there are RNA (UMI) counts for the number of times 2 chains (i.e. BCR…
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I am currently working on some fixes for the MultiQC reporting. While doing so, I wanted to update MultiQC version to its most recent, since the issue I submitted: https://github.com/ewels/MultiQC/iss…
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Hello, I've been instrumenting numerous QUIC implementations for performance testing.
In particular, I see [`quiche`] from CloudFlare implemented UDP offloading and GSO earlier this year. [[link]](…
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I would very much like to use MetONTIME for long read sequences and to proceed with 16S analysis.
However, when I run Launch_MinION_mobile_lab.sh, I get the following error and it stops.
In MinION…
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I'm using cutadapt 4.1 with Python 3.10.6 through conda.
I'm trying to demultiplex a dual indexed library with inline barcodes where the first 8 bp of each read are a unique barcode.
My command…
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Hello,
Is there a way to calculate the ram requirement for the program? I run the program on a cluster. The jobs are killed because of high memory usage. E.g. my lastest job exceeded 256 GB RAM usa…