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Hi,
I am trying to find a tool that can process nanopore data and identify and trim my PCR primers in the dataset?
How would I do that with porechop_abi? is that possible?
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Thanks for this nice tool @matdoering
I wonder whether it would be possible to specify the desired amplicon size. In my case, I want to amplify small regions of a bunch of genes (one per gene), bu…
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Hi,
Thanks for a great tool. I am playing around with genotyping amplicon data from Nanopore sequencing. I can get Straglr to call certain STRs but not others, and I wonder if I need to do somethin…
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### Checklist
- [X] There are [no similar issues or pull requests](https://github.com/taxprofiler/taxpasta/issues) for this yet.
### Problem
A simmilar issue of different file formats exists for am…
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Hi,
In version 0.15.0 seqkit amplicon only keeps one amplicon (the largest) per primer pair. I don't always care about the largest amplicon.
Would it be possible to implement the feature of kee…
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Dear Sirs, I have some problem about tmp file which was not created. I ran the job in linux server. There is some error in below:
passedQC_iF02_iR09.fasta contains 17 reads.
--> Low number of read…
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- primers initially
- amplicons would be a bonus
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Hi mate!,
Love your program it has become my default to go for clustering and consensus.
I have been using it with the error below sporadically poping sometimes, but recently it is happening e…
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Hi,
I have analysed ITS1 amplicons from fungal samples (leaf endophytes) using kraken2 and the PlusPFP-16 indices provided by Ben Langmead.
These amplicons were trimmed for PCR primer sequences, b…