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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?
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Dear all,
I did amplicon sequencing (16S) from woodchip bioreactors that were used to clean water contaminated with pesticides. I am very new to the field and I am still learning how to analyze the d…
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**Current term details**
```
Term name - 16S recovery software
Term ID - MIXS:0000066
Structured comment name - x16s_recover_software
Definition - Tools used for 16S rRNA gene extraction
```
…
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I am applying CNVkit to targeted amplicon sequencing data by following your command:
cnvkit.py batch -m amplicon -t targets.bed *.bam
Is this command using the default bin size of 200?
Some of …
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Hi, I found that for targeted amplicon sequencing data, the mutations called by Pisces 5.2.7.47 will mostly be flagged as 'SB'. Is it a property of amplicon sequencing data or primer specificity? how …
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**Describe the bug**
When trying to simulate reads with length greater than 400, I get an IndexError: list index out of range error
**To Reproduce**
Steps to reproduce the behavior:
` python gen…
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Good afternoon!
These two tools seem similar in some ways, and I would appreciate your feedback how how they differ in approach and intended usage.
>a rare read is erroneous if it has a neighbor…
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Hi I've noticed there're problematic sites added for V4 as well. Great work guys!
Just wondering if we can have an additional column for the particular setting (sequencing platforms, amplicon version…
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"Hey, thank you very much for this cool tool. I'm working on amplicon sequencing derived from public data with taxa at the genus level. According to the instructions, the only mandatory input is the a…
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Templates have been used to create the following files:
- protocol_sampling_CTD.md
- protocol_DNA_extraction_Sterivex.md
- protocol_PCR_16S_V4V5.md
- protocol_PCR_16S_V9.md
- protocol_sequencin…