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I'’m using the `isomiRs` Bioconductor package to analyze microRNAs. I’ve pre-processed the data with the `nfcore-smrnaseq` pipeline, and I’m working with the `export/{sample.id}_mirtop_rawData.tsv` fi…
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Hi,
I would like to analyze isomiRs by using isomiR-SEA ,mirtop and R-package(isomiRs).
Your quick start example to import isomiR-SEA files is
(and maybe your example of 'isomiR-SEA' is example …
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Hello, I had a quick question/clarification re: duplicate miRNA IDs. I noticed in the duplicates matrix there were a large number of miRNAs called duplicates due to the license plate but when you look…
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Dear miRTop colleague,
We are inviting you all to participate in an isomiR research project. We have been talking for some time about isomiR naming conventions. One of the potential challenges wi…
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Hi,
I wanted to analyze isomiR output using your isomiRs package. We already set up miRNA pipeline with sRNAbench, so I tried to convert from sRNAbench output to isomiRs input using mirtop.
The s…
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Hi all again!
cc: @lpantano @gurgese @ThomasDesvignes @mhalushka @mlhack @keilbeck @BastianFromm @ivlachos @TJU-CMC
I will start a issue column type at a time. Let's see if that makes easy to ge…
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Here I'd like to open the discussion on the use of the "star" symbol.
Originally, when we thought that only one arm of the hairpin was functional, the star symbol (_) was used to convey that this str…
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@lpantano @gurgese @ThomasDesvignes @mhalushka @mlhack @keilbeck @BastianFromm @ivlachos @TJU-CMC @sbb25 @phillipeloher
Hi all,
It will be a little long email, but please take 15 min to go over…
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_MIRFLOWZ-prepare_ execution fails for the isomiR annotation processing steps when `bp_5p` and `bp_3p` are set to `[0]`:
```console
Error in rule iso_anno_final:
jobid: 15
output: result…
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**Questions**
1) I have 61 samples, then could I get result from 61 samples? Specifically,
**is there a maximum sample number when running miRge3.0 ?**
The data size ranges from 700M to 1.2G …