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I have created .bam files from nanopore data using Dorado.
What script/command of Methylartist I shall use to complete visualization analysis?
db-guppy or ????
what is next step?
where should I us…
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Hi are there .bam files available for the spatial transcriptomic data?
Thank you
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I run the following code to load bam or bigwig files:
```
BamBigwig_to_chipProfile(signalFiles = dirBW[5:8], format = 'bigwig', testRanges = TSS,
style = "point", bin_si…
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Hi
It is great to hear the support for long-reads coming to kallisto I would like to introduce it to our pipelines
I have few question regarding running lr-kallisto on ONT dorado basecalled rea…
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### Is your feature related to a problem?
I attempted to run the workflow with the normal data coming from two separate (barcoded) sequencing runs. The two runs were basecalled (and demuxed) in two s…
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Thank you for htslib. We are planning to compress a lot of BAMs (~250K files) to CRAMs. We are concerned about possible file corruption and data loss. Therefore we plan to validate each CRAM has the s…
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#### Description
Add an option to the script to generate subset BAM files for screenshots and variant confirmation. This feature should ideally remove traceable information such as read names and B…
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gridss conda installation
jar file 2.13.2-gridss-jar-with-dependencies.jar
platform : Ubuntu 22
The code I ran
`gridss -t 20 -r ./reference/Ref/GRCh38.p14.genome.fa -j ~/tools/gridss-2.13.2-gri…
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Dear espresso development team:
Greetings~
Allow me to describe to you the problem I'm having: when using the command ESPRESSO_C.pl, I'm running the code as follows:
perl /home/zhaojiaxin/anacond…
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Hi,
I am interested in assessing differential splicing between two populations.
I got PE-50 stranded mRNA libraries, which were mapped with STAR, and I thought on using the output bam files to run…