-
CRISPR systems are classified into types based on which *cas* genes they have. The current [classification system](http://dx.doi.org/10.1038/nrmicro3569) (ping me for pdf) divides CRISPR systems into …
-
Hi! Thank you, the analytical workflow you provided is amazing and the most streamlined I could find for GESTATALT-like methods analysis.
I was just wondering at which point in the pipeline script yo…
-
Hello!
I just had a quick question. Looking through the documentation I am a little unclear if knock-knock can be used to analyze CRISPR NGS data generated using multiplexed PCR (e.g. multiple ampl…
-
**Analysis of tested guide RNA’s used for gene editing via CRISPR to predict optimal future guide sequences**
Despite the apparent simplicity of this sequence matching strategy to target the CRISPR…
-
@rasi, @gquarter has generated some results from her GO analysis using the splicing CRISPR screen data, see [here](https://cbl-gorilla.cs.technion.ac.il/GOrilla/zctdtiao/GOResults.html). Do you have a…
-
Hello!
I have successfully ran the program, but when I try to upload the Mapping_sorted.bam file into IGV, it asks for the index file ".bam.bai" file which is not there. When I try the sam file in…
-
Right now the paired counts are inner joined (the default behavior of `merge`).
https://github.com/sheltzer-lab/crispr-screening/blob/1a6f8c1cbe94433e4abfc02d47247ba92c21ade4/bin/extract-reads.py#L…
-
I am writing to you because I am collaborating in a CRISPR/Cas9 knockout screening analysis. We are using the GeCKO v2 gRNA library and we found that a huge number of gene symbols are outdated. I foun…
-
Hello,
There are several files missing from the distro for LongAmp such as longamp_delly_calling.sh and longampfigures_distribution.py.
Is there a reason these were left out?
Also is there some …
-
Hi Jan,
Thank you for such an amazing analysis tool.
I recently successfully ran the application on our Ubuntu server. However, we encountered a issue of connection. Sometimes I can connect the …