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I am going to be trying out `polyalign filtered`, and then `polypolish polish` on a 2.6Gb mammalian genome assembly soon (on a high performance cluster). I realise this is far outside the scope of wha…
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Hi,
How to speed up when we are annotating large genome (>=5Gb) using heliano ?
Can I split genome sequence into small pieces (5-10Mb) and annotate them independently ?
Best,
Kun
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Indexing onion/garlic genome failed since the genome is 16Gb and two chromosomes are longer than 2.1 Gb.
Does BWA has a limit for sequence length?
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Hello
Thank you very much for your great work! I run scAllele and got a vcf output. My cells are geneA exon5 knock out cells by CRISPR. But scAllele did not find any deletion in exon5 of geneA.
…
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when I ran large genome(44G) with trf,this program send follow bug:
`Loading sequence...`
`Error: Insufficient memory`
`Error: Error while loading sequence`
so , I try to use one chr(4G) to run, a…
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Hi Shujun,
I'm trying to wrap up some work on the Hesperis matronalis genome and am having a problem with panEDTA - it keeps timing out. I've cranked up the RAM to a terabyte and it still just runs…
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**Is your feature request related to a problem? Please describe.**
I am insterested in download a specific region of the genome. The[ web interface](https://www.ncbi.nlm.nih.gov/labs/virus/vssi) allo…
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Hi,
I found that gffread (v0.12.7.) can not deal with large chromosomes, we may find minus coordinates when using gffread to deal with such large chromosomes, for example, for a genome here https:/…
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Hello everyone,
I'm trying to polish a large genome (3Gb) with Pilon but I'm having problems with RAM. I read that some people choose to split the genome to deal with RAM, so I would like to try th…
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I generated a large similarity table from ~1 million genomes using sourmash'es branchwater and tried to cluster it with Clusty. Clusty didn't finish reading the input after 6 hours, while pyLeiden fin…