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Preferred Term: m6A-MTase sequencing assay
Synonyms: Fiber-seq
Definition: A DNA methylation profiling assay in which chromatin stencils are created by treating nuclei with m6A methyltransferase (MT…
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Hello,
I have another question concerning this pipeline.
The m6A accessibility and level are calculated using in vitro samples : do you think it is possible to estimate the m6A accessibility in a…
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Hi, I hava two problem in learning nanocompore.
1. The following files are provided in https://www.yeastgenome.org/strain/SK1 about reference file site mentioned in paper. Is the `SK1_NCSL00000000_S…
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Hi,thank you for your great tools for m6A modification analysis,i got a problem when i use the ‘countReads' function.I got this error " Error in countReads(samplenames = spnames, gtf = "./mm10.ncbiRef…
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Hi, I downloaded the respective FASTQ (SRR14765640-14765647) from Zhang et al. raw MeRIP-seq data (GSE151028) and generated the list of m6A sites using HISAT2 and MACS2 as described in the methods sec…
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Hello,
I recently followed the protocol of m6A sequencing which is published in 2013 (Dominissini, Dan, et al. "Transcriptome-wide mapping of N6-methyladenosine by m6A-seq based on immunocapturing an…
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Hi Prof. Zhu,
I tried to run my own data, but there is a error:
Make the TxDb object ... OK
Sorting and indexing BAM files with Rsamtools...[bam_sort_core] merging from 28 files and 1 in-memory…
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# Issue Report
## Please describe the issue:
Running dorado basecaller 0.7.0 with modification calling using direct RNA model 0.5.0 results in error: *** Error in `dorado': double free or corrup…
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Hello, Thank you for developing a great tool.
I have run DRUMMER with my data and obtained several output folders. I used the same command line for DRUMMERS for both sample 1 and sample 2.
However…
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Hi, I am working on the m6a identification on my ONT data. I installed NanoSPA according to the instructions. I can run the test data successfully and get the output files in the plus_strand folder. H…