-
Can I use this software on an already assembled partial mitogenome. The genome was sequenced as metagenomic environmental sample using Oxford Nanopore long read technology. Any help is appreciated
-
I'm trying to run some nanopore test reads through mothur but am getting stuck at the very beginning. I was going to follow your pacbio example so was trying to start with fastq.info. My data are conc…
-
Hello,
Would be possible to perform a SNP variant calling using Nanopore sequencing data? What parameters would have to be modified and how could a custom error model based on Nanopore be created?…
-
My name is Jemima and I recently started a PhD working on stool and vaginal samples from a clinical trial in SSA. I am currently working on sequencing the full 16s gene on nanopore platform. I have s…
-
Dear Dr Jue Ruan,
I am using wtdbg2 with the new nanopore Q20 reads. I tried setting -x ccs , but got a very fragmented and incomplete assembly.
May you please suggest which parameters should I t…
-
There are some issues with this training that I collect here to fix step-by-step.
* In the preprocessing part, all fastq outputs go into multiQC, but since multiQC only shows one output for identical…
-
Hello,
Is it possible for PsiClass to be executed on long-reads generated from PacBio and/or Nanopore? If not, is it possible to make quick modifications to the code to adapt to long reads?
Than…
-
Hi,
Trying to use nanopore reads as input using the latest version of breseq (thank you very much for developing this feature), however, I get the following error:
terminate called after throwin…
-
Would you recommend using CANU-corrected minion reads as suggested in this publication? https://pubmed.ncbi.nlm.nih.gov/32419382/
Is HLA-LA capable of producing good results from raw minion reads?
-
Can DIPAN be used in long-read transcriptomics such as nanopore reads?