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Hello,
We are doing high-throughput cloning of different gene variants and want to move the QC of our cloning products to Nanopore sequences. I did a trial run with a library of ~400 variants. The …
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Hello,
Thank you for making this program.
I am hoping to resolve some haplotype switch errors in my regions of interest, and I think Methphaser would be very nice to try out.
My regions of int…
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I am trying to create a consensus sequence (or consensus sequences for a few of the most abundant morphs) for the rDNA repeats of several mammal species that do not have existing rDNA reference sequen…
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Can I use this software on an already assembled partial mitogenome. The genome was sequenced as metagenomic environmental sample using Oxford Nanopore long read technology. Any help is appreciated
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Hello!
I would love your opinion or advice with our method, and I have a few questions about how UMItools extract with a regex works.
I have nanopore long reads with dual 18bp UMIs, one on eac…
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Hello Professor Li. It's a wonderful tool for 3D genome analysis.
Recently, I am dealing with the scNanoHiC data with bwa-sw and hickit, and I found that the haplotype phasing results were confusing…
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The usage report printed by "rgi bwt -h" shows the only available options to be for paired end illumina reads.
Is there any flag to feed it single ONT sequenced metagenomic reads?
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I'm trying to run some nanopore test reads through mothur but am getting stuck at the very beginning. I was going to follow your pacbio example so was trying to start with fastq.info. My data are conc…
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Hi,
I have been using NanoSim quite a lot lately. I did use it for simulation of nanopore reads or to control the quality of long reads with the read_analysis.py-script it works perfectly fine, ex…
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Hello. I'm working on T2T trio assembly, with approximately 90× HiFi reads, 115× ultralong ONT reads, and about 64× coverage of NGS reads from both parents. Below is the command I submitted:
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