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Dear tseemann,
I want to only annotate plasmid sequences from an assembly file (contig.fa). Can you please guide how can i do this?
Thanks
J.A
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![image](https://user-images.githubusercontent.com/17573404/74113401-77b19f00-4b69-11ea-8441-987488f39a86.png)
This is a very useful library, thank you for developing it! I am interested in using D…
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For plasmid you will not get a good result if you just use the default settings.
I would recommend getting GENBANK files (.gbk or .gb) of all the plasmids that are similar to your one.
Say you g…
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Hi @manulera!
I wonder what would be the best way to modify a DNA source when it comes with any sort of error. I'll explain:
Let's say I get a plasmid from AddGene, but I discover my aliquot has…
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This happens in the MYTK MoClo kit, where the sequence of the homology arms of 500bp does not exactly match that of the genome, having a few mistmaches. What to do in a case like this? Include `N`s?
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I have a genome contig of about 30 kbp that I did an annotation using RAST and I downloaded as EMBL and also as Genbank file to compare with a plasmid in the NCBI database. When I insert the EMBL file…
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Hello everyone,
I'm a student of Manu's and I'm writing some documentation for pydna. I'm now writing documentation for the gibson assembly page, and I had been recommended to ask for some useful e…
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Related to #136, can be reproduced with the same files found in there ([reproduce.zip](https://github.com/user-attachments/files/16993240/reproduce.zip)).
When using the `pcr` module in this case, …
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All plasmids are here: https://www.addgene.org/ASAP_Discovery/
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Hi Bakta developers!
Thank you for a great tool for plasmid and bacterial genome annotation!
I am using Bakta specifically to annotate the origin of replication. In some of my plasmid genomes, Ba…