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**Description:**
When attempting to generate molecular structures using the `polygon` tool, I encountered a `FileNotFoundError` related to the missing `fpscores.pkl.gz` file. The following is the c…
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I tested the same command with different two datasets, polyG still remains if the data is large (i tested 60M SE reads). polyG is still there and not be trimmed. fastp --in1 test_1.gz -g --poly_g_min_…
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Hi,
I want to detect the polyG in read tails and discard them instead of trimming .
can you add this feature to fastp ?
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Hello OpenGene team,
is there a way to know from the fastp logs how many reads were polyG trimmed?
Many thanks!
Jorge
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We use NovaSeq to sequencing and have polyG problem. Now we want to remove all reads in 2 paired-end files which has polyG length larger than such as 50 bases.
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I am part of a team doing targeted sequencing of PolyG loci. We have previously been using LobSTR for genotyping polyG sequences from reads prior to a consensus making step (see https://github.com/ri…
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is the following approach suitable to
modularize random ideal construction?
```
newstruct("TGenerator","proc fkt,list params");
proc randomVariable()
{
return ( var( random(1, nvars(basering)…
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Hi Guys,
I am using aDNA trim as follows:
`seqtk mergepe R1.fastq R2.fastq | adna-trim -p aDNA_trim_pe - > aDNA_trim_merged.fastq`
The data is a NovaSeq sample pre-processed with FasP to trim pol…
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Hi..
Finally I could managed to calculate the area of my polygons ( I had to fix geometries but finally it runs kakaka) for one of the months of my data but now I would like to run the script for …
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Given a sequence with 5' adapter, eg **ALONGADAPTOR**sequence, if sequence is low quality in the end, or has polyG, cutadapt will trim this sequence into **ALONGADAPTOR**seq (*1st case*) or **ALONGAD…