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Hi,
Does Melon accept assembled contig data produced from short-read sequencing data using assemblers (e.g., MEGAHIT, metaSPAdes...) ?
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Hi,
I am hoping to perform transcriptome assembly using both nanopore long read sequencing data and illumina short read sequencing data. It appears RATTLE only permits the use of long read sequenci…
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Hello! Thanks for your helpful tool!
I am trying to assemble long-read sequencing data with short-read polishing. My long read data (nanopore direct RNA sequencing) are from the animals under 25 de…
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**Is your feature request related to a problem? Please describe.**
When users polish long read assemblies with short reads, they tend to believe that the new consensus is always better than the origi…
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Should the sequencing depth of different datasets and batch effect issues be considered when merging RNA-seq data from different cohorts for input?
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Hi,
We'd like to request a new term as part of the CFDE project for Kids First DCC.
new term label: linked-read sequencing assay
parent: DNA sequencing assay, OBI:0000626
definition: A DNA seq…
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Dear Dr. Sean,
I hope this message finds you well. I am writing to express my admiration for your recent publication in Current Biology titled "High viral abundance and low diversity are associated…
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### Description of feature
Due to the high fragmentation of ancient DNA data, there is not much gain in using long-read DNA sequencing methods for assembling ancient DNA samples. However, many ancien…
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The kma seems to be ran with default parameters which means that the identity cut off is about 20% for sequence identity. Why is there custom no cut off for KMA?
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Hi,
I am trying to assemble the genome of a worm, and I was hoping that minipolish would detect the mitochondrial genome and output it in the gfa file.
I have sequencing one of my worm species a l…