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Preferred Term: m6A-MTase sequencing assay
Synonyms: Fiber-seq
Definition: A DNA methylation profiling assay in which chromatin stencils are created by treating nuclei with m6A methyltransferase (MT…
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Dear Developers, hello!
There are some projects using PacBio single-molecule long-read sequencing to analyze full-length transcriptome, but the raw data is in a bam type file and has to be changed in…
wu116 updated
1 month ago
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It's OK In prod (e.g. here https://docs.lamin.ai/scrna#populate-metadata-registries-based-on-an-artifact)
I just saw it in a notebook from @sunnyosun
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UMIs are mostly used to track single molecules and enables us to distinguish them amongst the different reads we got from an experiment. With them, we are able to not mark as duplicates identical read…
Poshi updated
3 months ago
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Hi there, it is a great work of Bacmethy. I'd like to know if Bacmethy only supports Single Molecule Real-Time（SMRT）Sequencing data or it is compatible with Illuminasequencing data ?
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Hi,
First of all, thanks for making STARSolo.
Is there any way to get HDF5 as output from STARSolo pipeline after mapping/counting 10x droplet data?
Or any other way to get each individual tran…
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### Enzyme, ADAR (proof-read and correct mistakes in RNA)
[**ADAR editing enzymes** are found in all multicellular animals and are conserved in sequence and protein organization. The number of ADAR g…
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https://www.biorxiv.org/content/early/2018/04/28/310458
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https://slyli.cn/posts/2021-07-002.html
靖康耻，犹未雪。臣子恨，何时灭。驾长车，踏破贺兰山缺。壮志饥餐胡虏肉，笑谈渴饮匈奴血。待从头、收拾旧山河，朝天阙。
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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?