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## Problem Summary
Several values in HuBMAP metadata require consolidation or mapping updates to align with the current standards. The issue affects two categories:
- **Granularity Mismatch Mapp…
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library(irGSEA)
library(Seurat)
Loading required package: SeuratObject
Loading required package: sp
‘SeuratObject’ was built under R 4.3.0 but the current version is 4.4.0;
it is recomended that …
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Hi,
I was looking into the vignette script you provided for the Thrane melanoma dataset and especially how to do the DE analysis
using the seurat_cluster_DE function (http://www.ezstatconsulting.c…
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## Design
## `X` (Matrix Layers)
The data stored in the `X` data matrix is the data that is viewable in CELLxGENE Explorer. It MUST be encoded as a [`scipy.sparse.csr_matrix`](https://docs.scipy…
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i analysis the scrna-seq of two conditions and used the DotPlot functions to show the average expression levels in different conditions. however, i found there is an opposite trend between the DotPlot…
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Hi
I have three single cell RNA seurat object to integrate. Each one is separated rds file. Actually I didn't find how to deal with this situation cause the tutorial load the processed data.
So my…
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Hi,
I am recently using the ProjectData method,I noticed that there was a missing cluster in fulldata
, as shown in the following figure,there is cluster10 in seurat_cluster.sketched, but not in fu…
lilwo updated
1 month ago
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### Analysis Environment
- [x] Installation of `scverse` tools
- [ ] #19
### Analysis Steps
- **Preprocessing**
- [ ] #9
- [ ] #14
- [x] #4
- **Cell typing**
- [ ] #16
- [ ] #3
…
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Hi,
I'm trying to use the MetaspotsByGroups function with multiple variables from meta.data, including "case," "batch," "region," and "distance." However, when I include the "distance" variable, I …