-
Hello Team,
I simulated a shotgun metagenomic Nanopore dataset using NanoSim, containing:
65% bacterial sequences from 32 bacterial species
35% human sequences
For profiling, I created a Sylph…
-
Hello,
I built a custom bacterial database following the instructions from the kraken2 manual on github (https://github.com/DerrickWood/kraken2/blob/master/docs/MANUAL.markdown). This went successf…
-
Hi,
I have created viral database and when aligned my samples using Blastx only one subject is showing and it is bacteria. I have aligned more than 30 samples but only can see one subject id (WP_0159…
-
Hi there, when i use SMTa for microbe analysis in visium data, i noticed that the nt database is too large and time consuming.
![114027](https://github.com/affinis/SMTa/assets/36031820/17f79c24-88b0-…
-
I have built Taxonomy from bash taxonomy.sh reference.fa
my reference sequence is around of size 372 GB
when the command is run
cargo run --release -- -s bacterial_alignment.sam -m bowtie2…
-
Dear KrakenUniq team,
I have a theoretical question - I was wondering if you could suggest something here. I am using KrakenUniq to classify bacterial reads from RNA-seq experiments. The database …
-
I am working on processing bacterial 16S rRNA sequences with DADA2. I would like to classify these sequences with the newest update of the RDP database (version 19), but I have yet to find it formatte…
-
Hi there! I'm attempting to perform a homology search for a single gene (a bacterial protein) across all sequenced bacterial genomes to estimate the % of species that carry the gene. Is there a way to…
-
Hello, I have encountered some tricky problems.
1. I can't download your kmer database from Google drive in the HPC cluster under terminal. Although I tried several google drive command line download…
-