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Hi!
I have been experimenting using Milo to analyse mass cytometry data. The data I try to feed into the Milo pipeline has been clustered using FlowSOM, and I am using UMAP for 2D projection. My SC…
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Hi!
First off, thank you for creating such a great tool!
I'm working with a dataset that includes over 200 markers, and we’re looking to identify the top 5 or 10 markers that define each cluster…
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Hi,
I am trying to apply a pseudotime analysis to flow cytometry data. These data a processed through a pipeline similar to the CytofWorkflow (Nowicka et al., 2017) and we generate sce objects tha…
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We need to determine an appropriate way to preprocess the data, since protein abundance is not a linear function of the raw measured intensity. In the original Bendall et al. paper (http://www.science…
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For checking:
```
PMID:1482118 | Genetics of the fission yeast Schizosaccharomyces pombe.
PMID:7011176 | Genetics of the fission yeast Schizosaccharomyces pombe.
PMID:9439701 | Identif…
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Hi,
I'm interested in using SPADE for non-cytometry data and have followed the recommendations in the [FAQ](https://github.com/nolanlab/spade/wiki/FAQ). I have a few questions about the process:
1. I…
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Howdy. Shouldn't there be a term for "flow cytometry antigen panel", defined as a group of antibodies tagged with different e.g., fluorochromes or rare earth elements, wherein that group is used toget…
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I was wondering if parade was able to process quite easily histo cytometry application (with dataset for different condition/activation on Omero) if the segmentation results is given as mask (to ext…
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The current catalogue record [rewrites the CSV index of tagged images](https://github.com/NERC-CEH/plankton_ml/blob/main/scripts/intake_metadata.py) to add an absolute path to the location in s3 - but…
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Hi,
I'd like to use pyVIA with flow cytometry data, could you add a tutorial for this kind of data?
Do you have some suggestion about parameters to set?
Thanks you in advance.
Simone
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