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Hello,
I'm analyzing RNA sequencing data of samples from every chromosome, and I'm trying to gauge the expected duration for the Intron Clustering process. In my recent analysis, processing 10 samp…
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Hi,
I found some extremly long introns in spaln results (when mapping protein sequences to genome), some are ~1Mb, is there a way to limit intron length when running splan?
![image](https://user…
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Hi Everyone,
I was successfully able to run TEtranscript using these codes in command line:
```
singularity exec tetranscripts.sif TEtranscripts \
-t /home/tud03125/pipeline/mouse_liver_rnas…
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**Are you using the latest release?**
funannotate v1.8.17
**Describe the bug**
When I run funannotate predict I get an error
[Oct 15 05:17 PM]: Training Augustus using BUSCO gene models
Error: …
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Hi authors, great paper just a couple of nitpicks in the paper that might make it a little clearer.
1.
To use it you have to provide a set of contigs resulting of a de novo transcriptome assembly…
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I am trying to use rmats2sashimi and for some reason, I am encountering a similar issue that was reported before and commented on October 8, 2021 Issues # 72 and 115.
usage: rmats2sashimiplot [-h] …
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## The need
In the case of introns (eukaryotes and some of their viruses), the estimated genomic redundancy can be highly inflated, wrongly indicating that the quality is bad.
## The solution
…
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Hi everyone,
I am trying to analyze some transcriptome assembly I created via Trinity. Considering the final nucleotide sequence is called .cds, does transcdecoder exclude introns? Because I am loo…
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Dear @emmanuelparadis,
thank you for maintaining the package pegas.
I'm a vet and doctoral student and I'm currently working with NGS data obtained from parasites. The "problem" is, that the seq…
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Hi there,
I was testing LiftOff and LiftOn to see which one is "best" for genome annotation. I was looking at some RefSeq genomes, lifting the brown-headed cowbird and red-winged blackbird onto the…