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Hello Sebastian and co.
Thanks a lot for designing this nice package to understand the nature of TCR repertoire and potential expansions. My goal with the current airr data I have is to compare dif…
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Dear all,
I am running into an issue with the metaclustering from the **run.flowsom** function. Whether using an automatic number or setting a number, I get one big metacluster with 99% of cells, a…
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Hi, i am trying to use tf-modisco to find motifs, but I encountered the following error during execution:
```
MEMORY 3.652018176
On task task0
Computing windowed sums on original
Generating nul…
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Hello Sophie!
Thank you for a great package!
I have a question regarding the optimization of flowSOM clustering.
I set a seed, used an 11x12 grid, and varied the nClus parameter to test a dif…
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In R console I call FlowSOM() to load an FCS and apply clustering and metaclustering and then call SaveClustersToFCS() to save the modified file.
However, SaveClustersToFCS reports an error to me (…
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Hi everyone,
following a brief discussion with @burtonrj in #71: There is a Python implementation of `FlowSOM` by the original authors (https://github.com/saeyslab/FlowSOM_Python), which offers a c…
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Hello again :),
I have been running into a weird issue where I specify the number of clusters as e.g. 20, and it's running flowsom with nClus=20, the plot for the CV looks ok, but when it's doing t…
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Hi, I'm having troubles changing the file after running FlowSOM. This has previously worked, but when uploading to cytobank, I am now missing the new cluster and metacluster channel. I'm following the…
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Hi,
I hope you can help me with this. I am trying to analyze flow cytometry data acquired in a Cytek Aurora. I have 230 files, but I am using only 12 files from 4 different batches to see if I can …
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Hello. I ran into a problem with using multiple `new_scale_fill`. It doesn't seems to work for me when I used more than two. Please see the example belows.
```set.seed(445)
plot_data