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Here are details regarding barcodes into PCR products once general primer pair is determined to be viable
https://d.pr/f/K2we5f
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In order to support the automatic upload of sequence data from multiplexed high-throughput sequencing (PacBio protocols in our lab specifically) we need to be able to add Multiplex Sequencing Run reco…
silby updated
5 years ago
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Hi @joybio thank you for your package.
In the results folder of multiPrime for the CDS test data, there are two final max forward and reverse primer from just one cluster.
Is it expected that the …
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Hi
It would be cool if there was a wiki section that would include the entire approach (including the wetlab part) of training new models. e.g. what would be the best experimental design to train m…
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It would be nice if vsearch could search for PCR products given primer sequences, as the usearch `-search_pcr` function does. The `search_oligodb` command probably does the heavy lifting and would nee…
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suggest to index host-pathogen interactions from PREDICT PCR TestsGlobal Health Security in Development (GHSD)
[View Data](https://data.usaid.gov/Global-Health-Security-in-Development-GHSD-/PREDICT-P…
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Example:
`(1/1) Processing cutsite: Genome:ensembl_GRCh38_latest, Chr:8, cut_coordinate: 21919773
[Warning] No primers found, relaxing criteria, iteration=1
[Warning] No primers found, relaxing c…
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We need to do a bead size selection clean up to remove primer dimers from our pooled PCR product for ITS2. Details of the amplification and prep for these samples are in my [notebook post](https://ahu…
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I am working on a CRISPR data treated by two sgRNAs and sequenced with targeted sequencing. The distance between the two sgRNA is 3000bp which is much longer than amplicon. The targeted area is the w…
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see https://github.com/ietf-rats-wg/draft-ietf-rats-corim/wiki/Composite-device-description-using-domain-membership-triples