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https://mp.weixin.qq.com/s/Oq_SUfuoaa6x0zt4y3jEPg
ixxmu updated
7 months ago
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Hello again!
I wanted to migrate from my old Cutadapt 3.7 to the shiny new 4.4, but after some testing, I saw that I was getting very different results, where the only difference is the cutadapt vers…
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Hi all,
I wish to compare my sequencing results of stool samples with the data available in the Human Microbiome Project. What I want to do is to calculate Unifrac distance between all the samples an…
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Hi, I have tested the gut 16s amplicon of a lot of host such as duck, blacksoldierfly,silkworm, housefly, et .al and some enviromental metageomes sequnced on illumina by paire ends. Unfortunately the…
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I am running the latest version of Cecret on paired-end fastqs as follows:
nextflow run UPHL-BioNGS/Cecret -profile singularity -c configs/sarscov.config --medcpus 70 --maxcpus 100 --reads reads --re…
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Hi,
I'm facing a curious problem. I've sequenced twice a sample : first with the R9 flowcell version and second with the R10 flowcell version, and it looks like I'm loosing some information in the…
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I am using header name from hashing ASV sequences to integrate ASV table from different datasets. I found that there were duplicated ASV sequences. Why?
Here is my lotus2 command:
lotus2 -i $PWD -…
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Hi,
I have been trying, unsuccessfully, to run CRISPResso2 pooled with mixed-mode, as according to the documentation.
I noticed that there are tests for CRISPRessoPooled in "amplicon mode":
…
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貼吧活動：(請查閱 [SARS-CoV-2 Timeline by 2020.02.21](https://github.com/agorahub/_meta/blob/agoran/theagora/sari/Memorandum_2020-02-21_SARS-CoV-2-Timeline_Nathan.pdf?raw=true), by Nathan :cloud: )
- Colla…
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Hi,
two questions:
1) at the end of a run it'll say something like : 1201/4000 sequences assigned in groups for sampleX (30.0%)....
where did the remaining 70% go? is it that they failed to…