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Hello there. I am attempting to use trio binning. I have ont long reads for the F1 sample and illumina novaseq paired end fastqs for the F0 parental datasets. However, when I run canu it doesn't separ…
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We would like to assemble and haplotype a tree genome for which we only have the maternal genome available. The father could be any surrounding tree.
How could we generate the yak paternal file usin…
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Hi,
I would like to thank you for your continuous great work with the SQM pipeline, the latest release has some great additional features which are very relevant for my current work. I do have a fe…
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Hello,
I'm trying to use MUFFIN with nanopore and Illumina data but the error :
`N E X T F L O W ~ version 20.07.1
Launching RVanDamme/MUFFIN [fabulous_colden] - revision: 5805e280b0 [master]
NO…
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hello Surgey,
I have 30x of HiFi reads with which I would like to use by running Trio-canu for a Haplotype-specific assembly. Yet I don't have the trio information. However, by other approaches, I ha…
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Hi,
Thanks for the recent overhaul to the software. I was wondering if it is possible to run MOSCA only on MT data without MG data? I have some MT data simulated with Polyester and art_illumna (whi…
bwu62 updated
3 years ago
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Hi...
I am running SqueezeMeta and got an error at step 14
The whole error message was as follows
"Error running command: perl /home/anil/SqueezeMeta/bin/MaxBin/run_MaxBin.pl -thread 12 -contig …
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Dear developers,
Our group is working on a soil metatranscriptomic project with a quite large amount of samples. We plan to use the merged mode. Specifically for this kind of project, is there one …
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Hello Canu team,
I used Canu v1.8 to assembly PacBio reads generated for a fungus (36 Mb in size). The total reads are 133 Gb. I wanted to determine the minimum number of reads for good assembly, s…
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We have a trio sequenced on PacBio Sequel II V6 with CLR chemistry. 30-40kb reads and over 100X coverage for each. The independent FALCON-UNZIP assemblies are over 2mb N50. Genome is 876Mb, 10/20 chro…