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Hi @gbouras13,
Thanks for your wonderful tool. I noted that there were two choromosome contigs in more than one 'chromosome.fasta' files following hybrid assembly. I included the `--keep_chromosom…
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Using various strategies we can determine the expected size of an analytical PCR amplicon based on the parts list. Options for this:
1. Crude, but low user demand: assume mID-derived part lengths a…
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Hi @gbouras13 ,
I use hybracter to assemble bacterial genomes and plasmids from long read sequencing. I want to assemble single plasmids from long read sequencing but hybracter does not work with o…
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Hi, I would like to use lentiviral plasmid barcode libraries for lineage tracing based on an approach called STICR that was developed in a Nature paper last year. I have ordered two plasmid pools from…
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As an enhancement request, it would be nice to have the possibility to choose from more pre-existing configured vectors.
For now only 6 pre-configured vectors are available, it would be nice to ha…
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Because we don't currently have a good way to handling the import of the OpenYeast plasmids, they are appearing in the FASTA for synthesis. As such, any synthesis run needs to exclude them.
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kraken-build --standard --db $DBNAME
Only build Bacteria and Viruses, plasmids and human are not downloaded.
Not sure this is a bug or standard feature.
kraken-build --download-library plasmids --db …
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"If you have made functional modifications to the commercial plasmids you obtained, MSKCC would generally consider these new, modified plasmids to be their IP. We always carry out the MTA with the le…
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Hub plasmids get marked with a black star in the visualisation, and there's a toggle button that removes them, but any hub plasmids found in later iterations are black circles and don't get removed in…
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