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https://github.com/moka-guys/automate_demultiplex/blob/f9c5389ef9abb274076b437dea4ffb59ca5e1fa3/demultiplex.py#L326
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Hi, I have scTCR fastq and hashtag fastq of 10x Genomics. We don't have scRNA-seq fastq. Can we demultiplex scTCR data?
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Do not convert to capitals. this test would not stop the script failing downstream (path to samplesheet will be incorrect when checking the validity of the samplesheet and demultiplexing itself)
http…
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I have the same error for all the samples:
IPyradError( error in cutadapt -a AGATCGGAAGAGC --quality-base 33 -q 20 -u 5 --minimum-length 35 --max-n 4 --trim-n --output /home/avaldes/floragenex2020/p…
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**Improvement Description**
Python has [`multiprocessing.pool.Pool.map`](https://docs.python.org/3.6/library/multiprocessing.html#multiprocessing.pool.Pool.map) which lets you "spin up" processes, an…
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Hello Dr. Borcherding,
I'm a medical student working with single-cell RNA data from tumor-infiltrating lymphocytes. The output file our lab is currently working with contains a variety of patients'…
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I had a 10X run on the novaseq last week and I am having some issues with the demultiplexing. It looks like almost everything worked but there were only fastqc and multiqcs generated for the undetermi…
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Hi Seurat team,
The `HTODemux` function works great for datasets where each sample is labeled with one HTO. I was wondering what's the best way to demultiplex samples labeled with 2 or more HTOs?
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naity updated
3 years ago