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# dada2 pipeline for metabarcoding (CO1)
Thanks a lot for your dada2 pipeline, this is really cool. Our research group has been using it for our human microbiome projects. This is really cool.
We …
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'Omics terms can be difficult to learn. Having them described in a single page may help new comers to overcome this barrier quickly and transfer knowhow that may be a pain to transfer through more for…
meren updated
2 years ago
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Hi,
I have managed to install the Anacapa container and run the 12S example successfully.
Now I wanted to to run my own data but I am not sure I find all the options that need to be customized.
…
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Hi
I am try to convert a hdf5 biom 2.1 file using `biom convert -i data.biom -o table.from_biom.txt --to-tsv`
I checked that the file validates:
```
(.venv) me@12345 biom-test % biom validate-t…
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Hello @zachary-foster
I attended your workshop at the Phytobiome Conference in Montpellier last December and I managed to realize several comparing heat trees (using a phyloseq object). Again, tha…
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From attempting to recreate the figure from the underlying data (and my own analysis of the FASTQ files), I think there is a misplaced False Negative annotation on Figure 3. I do not believe this chan…
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Hi,
I would like to calculate the differences between two groups of metagenomic samples using the compare_groups function.
My concern is that I have a low number of samples in each group (4 vs 3…
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Hello,
Using trimming methods M1, my trimmed reads are too short to overlap and an erroneous contig is created (see below numerous degeneracies). Relaxing M1TrimmingCutoff to 0.0002, the merge is p…
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**Submitting author:** @daylinmorgan (Daylin Morgan)
**Repository:** https://github.com/brocklab/pycashier
**Branch with paper.md** (empty if default branch): joss
**Version:** v2024.1006
**Editor:** …
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Hi @gjeunen,
I would like to extract the amplicon regions from the same database using the insilico_pcr and pga functions for a large number of primers. I tried setting up a SLURM job to do this, …