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Thank you for developing such an excellent tool as semibin2, which performs exceptionally well and can generate a large number of high-quality MAGs.
Therefore, we are interested in applying semibin…
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Hi Alex,
I got the same error "FATAL ERROR in reads input: quality string length is not equal to sequence length" in a case recently. Previously, I have processed the fastq files with the same scri…
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```
[E::faidx_adjust_position] The sequence "2" was not found
ValueError: reference sequence for 'b'2'' (tid=1) not found
Exception ignored in: 'pysam.libcalignmentfile.__advance_samtools'
T…
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### Need
As a clinician I want high quality analysis, without false positive variant calls. At the moment in the UMI workflow we are not doing any quality or adapter-trimming. Not doing quality-trimm…
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I'm trying to alter the "mixed sites" qc rating, but I'm having trouble figuring out how.
The pathogen.json file and the documentation on Github (both pictured below) only mention a threshold of 1…
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`type_bug` | by Berserk Cyborg
___
See topic [http://forums.wz2100.net/viewtopic.php?f=51&t=12900](http://forums.wz2100.net/viewtopic.php?f=51&t=12900) and c89320c2d5ef7bb2262ce3e2659cdd58e2fb…
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Hi,
I have a dataset of NSCLC tumors that have both been whole-exome sequenced and RNA-sequenced. I have some more samples for which I only have DNA so I wanted to use ExTRECT to predict the T cell…
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PASTA should ensure download URLs are properly encoded before attempting the download.
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I got the error when running fastp on `sample.fastq.gz`:
ERROR: sequence and quality have different length
Could you help me resolve the issue? Thank you.
**My command:**
```
fastp --in1 "${R…
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Hi, thank you for the great tools! I am having some issues with merging reads across sequence runs. I am working with PacBio ITS sequel II data. I've run the workflow through the DADA2 algorithm by in…