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We used MASURCA 3.2.6 for genome assembly but found no fasta file for contig sequences at the end. Only final.genome.scf.fasta appeared in the CA folder. Is this normal? Below is the content from the …
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Any plans to output your actual contigs in GFA?
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I've tried to compile jellyfish and quorum. But it didn't work. So, I downloaded all packages from github specific directories and compiled everything. In the end, a error message for SuperRead compil…
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Hi,
I'm running `unitig-caller` in population mode to by used by `pyseer` for GWAS (following the documentation).
I don't have assemblies for all my samples (~250 samples), so I'm using the raw re…
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I finished running BGREAT and end up with a path file
How can i use GGmap to utilise path file which is from BGREAT? Do you example of using GGmap?
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Hi
We are evaluting the quality of raven assemblies using Nanopore V14 raw reads and we noticed errors close to window borders that we belive may be caused by racon. More specifically, after mapping …
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Hi,
I have a human genome that has a ~28 Mbp inverted duplication (invdup) on one of the haplotypes. This invdup region exists 3 times in the genome: once on the paternal chromosome, twice on the m…
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Hi
im trying to make hybrid assembly for Ecoli using MiSeq and Nanopore data in Masurca. currently i m getting two errors marked in Bold bellow. my MinION fastq files should be OK, no idea why im …
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I have a similar issue to the one described here: https://github.com/chhylp123/hifiasm/issues/442, but would like to understand it better. I run
`hifiasm -l 0 -o vptaxbacc_bk34-6 --seed 123 -t 48 -f…
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Hi I am using masurca (v3.2.6) assembler for assembly of plat with 1Gbp genome and I can observer quite high memory usage in command assembly.sh in line:
`create_k_unitigs_large_k -c $(($KMER-1)) -…