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"In terms of diagnostic terms for prenatal medicine, I took a look at the second link. Some ideas:
- [x] Imaging modalities used in the prenatal setting include both ultrasound (including echocardiog…
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I'm having a hard time understanding the documentation of `UMI_cor = 2` in `sc_gene_counting()`
> correct UMI sequencing error: 0 means no correction, 1 means simple correction and merge UMI with d…
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Dear Alex Dobin.
First of all, thanks for this fantastic tool and all your work during these years to update it.
Recently some colleagues attended the conference Biological Data Science 2022 and…
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I have been working with single cell sequencing data on R, where the data pbject is a seurat object, containing genes and cells in an expression matrix. I want to visualize expression of 2 genes in 13…
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I have run 48 cases of target enrichment sequencing of IHWG standard DNA and analyzed by PING.
All cases output their KIR allele in allele caller except 2DS1, 2DS2, 3DP1.
Although these samples have…
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**New term details**
For us to assess a new term request we require the following details:
```
Term name - relative amount of nucleic acids in the gradient position
Structured comment name - gradi…
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Hi alexdobin!
How is Sequencing Saturation calculated?
From the bam file of STAR, if the count of CB+UB+GX is unique, consider it as n_ deduped_ Reads, count all CB+UB+GX as N_ Reads, finally calcul…
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Hi,
First of all, thanks for making STARSolo.
Is there any way to get HDF5 as output from STARSolo pipeline after mapping/counting 10x droplet data?
Or any other way to get each individual tran…
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Hello,
I am interested to confirm an allele frequency for a SNV in BRAF gene using amplicon sequencing data. The length of amplicon is 271bp, and paired reads are 101X2. So there is a gap between t…
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Hi!
I have mapped my sequencing reads against the [IGC database](https://db.cngb.org/microbiome/genecatalog/genecatalog_human/) (nucleotide sequences), and wanted to run Omixer-RPM using the [GBM D…