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I am doing 16S rna amplicon based ONT sequencing.
My target region is only of 1kb to 2kb in length.
Per sample reads is around 50K.
Will HEERO give me good error filtered estimates ?
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Hello,
I'm running a Mini 16S bought as a *LSC Smart Connect*.
It is a device running firmware 4.x on which there are some differences like no http port at startup and requires `ppsFactoryTool.t…
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能不能实现没有diff不默认都渲染,加个查看更多什么的,避免全部渲染性能差,我得文件大概150kb 12000行渲染要16s,希望添加个加载更多~
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When updating the wrapper for the bracken data manager on galaxy, we found that in the docs of https://benlangmead.github.io/aws-indexes/k2 the bracken DBs are referred to as `All packages contain a K…
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Hi Noam,
I was using the lastest Scripts,but failed.
**Command**: sh 5R-master/5R_linux/run_main_5R.sh PATH_TO_RUNTIME ./example_fastq ./GG_5R GreenGenes_201305 ./example_results/5R_SMURF_exampl…
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The column remaining code here (https://github.com/biocore/metagenomics_pooling_notebook/blob/f573aaeee992afb7c570546d6624fd188110d70c/metapool/prep.py#L702) is either 16S or not. Will this silently r…
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I got the following message when running predict_traits.py:
predict_traits.py -i ./genome_prediction/format/16S/trait_table.tab -t ./genome_prediction/format/16S/reference_tree.newick -r ./genome_pre…
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Hello,
I am using kracken2, bracken and 16S_SILVA138_k2db to generate species and genus counts.
I am having trouble generating the bracken reports where I keep getting this error -
_Error: no…
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This issue is purely documentation for connection of 16S monitoring boards and the DIYBMS v4.X controller.
Cabling is similar to the single cell monitoring modules.
**DO NOT CONNECT TX TO TX O…
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Hi, I am trying to learn the error rates using DADA 2, i was able to do it for forward sequence, unfornutaly, fot reversed it always shows
"Error rates could not be estimated (this is usually beca…