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https://slyli.cn/posts/2021-03-002.html
靖康耻，犹未雪。臣子恨，何时灭。驾长车，踏破贺兰山缺。壮志饥餐胡虏肉，笑谈渴饮匈奴血。待从头、收拾旧山河，朝天阙。
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Hi,
I have got a reference genome which I want to polish with more than just one sequence file.
Basically I've got a reference genome which was generated using PacBio and then I've got 50 whole ge…
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Lets decide how we go with this.
I think it makes sense to test current assembly with real data that needs analysis to gauge status.
I sugget two ways forward -
More PacBio or Nanopore....
sr320 updated
6 years ago
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Dear bcbio developers,
For some of the species that we work on we are now starting to receive significant numbers of long read sequenced samples.
The samples are sequenced using nanopore, PacBio …
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- Rename output file csv -> tsv
- Echo parameters in stdout (useful for debugging)
- Perhaps add option for verbosity, using tidk on pacbio data results in huge output to stderr
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Hi!
I'm trying to run masurca with Illumina pair end libraries and pacbio long reads.
Here you have my confog file:
DATA
#Illumina paired end reads supplied as
#if single-end, do not…
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- Pacbio data
- Download
- select chr 11 and 20 with samtools
- chr11 >20kb
- chr20 >10kb
- start with chr11
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How do I use BString / BStringSet for values > 127?
PacBio data uses 0:255 ranges for fp/rp/fi/ri tags. I am trying to use BioStrings to work with these tags.
But I ran into trouble, for values …
gevro updated
5 months ago
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Is there a way to run NCLscan on non-paired-read data (e.g. PacBio output)?
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I'm trying to run some nanopore test reads through mothur but am getting stuck at the very beginning. I was going to follow your pacbio example so was trying to start with fastq.info. My data are conc…