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- [x] Trim adapter content
- [x] STAR alignment
- [x] Transcript quantification
- [x] Differential expression analysis
- [x] WGCNA
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Dear @smorabit,
Thanks a lot for all your help with my questions regarding hdWGCNA.
I was wondering if you have any advice on how to get fewer, but more specific modules.
Many thanks,
Paria
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Hi!
I would like your help. I have read your paper titled "Gene expression variability and the analysis of large-scale RNA-seq studies with the MDSeq" which is a excellent work. So I plan to use the …
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**Error**
Hello! Thank you for creating such an awesome package!
I used seurat assay 5 to perform hdWGCNA analysis. All function worked well until ModuleEigengenes function. When I run ModuleEigeng…
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I'm running the basic hdWGCNA pipeline using a set of large Seurat 5 objects that were generated using BP Cells to allow storage of large expression matrices on disk rather than in memory.
When ru…
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**Describe the bug**
When I use MetaspotsByGroups to treat my seurat_obj, It reports error. Looks like follow:
```
Not validating Seurat objectsNot validating Seurat objectsNot validating Seurat …
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Hi I am trying to run findModules(), but I keep getting this error. Have tried to change parameters with
pyWGCNA_adata.findModules(kwargs_function={'cutreeHybrid': {'deepSplit': 0, 'pamRespectsDen…
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**Describe the bug**
After running the MolduleConnectivity function all the Kme values are 0. I do not get any errors. I have me and hme values.
**Steps to reproduce**
I am following along from t…
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## Issue description
In d008513af25f264accdbd4f496bfe13803e28190 building of language specific packages was disabled on hydra. Now that faster hardware is available (https://grahamc.com/blog/an-epy…
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尊敬的老师:
您好!
我输出的结果压缩包要么只有预处理的结果,即两个excel文件;要么显示一直在运行。不知道出现了什么问题,烦请您解答。多有叨扰。
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