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Hello,
I have a V3-V4 16s DNAr metabarcoding dataset of 68 samples with a read depth of 400-900K reads/sample. Samples were sequenced on a NovaSeq 6000 PE250. Primers were removed with Cutadapt, seq…
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Hello!
I am working on a COI metabarcoding project for animals. I currently have two database files, one fasta file containing all sequences and the other txt file containing taxonomic information …
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I am interesting in post-DADA2 clustering of ASVs for more general animal biodiversity questions at the species or genus-level using, in this case, the CO1 marker. While the ASVs are very interesting …
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Dear Dr Callahan,
I hope this message finds you well.
Thanks a lot for all the work and development you have done with this package. It is very useful for my PhD project, which focuses on eDNA…
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Hi,
I have been wrangling some metabarcoding MiSeq data using dada2 and otu clustering to help simplify the search for recombinant sequences. I have managed to run through my make-shift workflow, …
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Hello
I am working with metagenomics data that was isolated from from plant under two different treatment.
I am using squeezemeta for the analysis and I want to further proceed with SQMtools for do…
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Hi,
I'm starting to work on a DNA metabarcoding project and I was following the amazing tutorial from Devon O'rourke to build my COI reference library from BOLD (https://forum.qiime2.org/t/building…
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Hi Dominik,
having the issue currently that no xlsx file is being written for my results.
Using boldigger2 v1.0.6 with python 3.12.1.
I have a fasta file with 1,773 COI sequences. After rough…
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While this benchmark isn't currently attained by similar software (e.g., https://github.com/zdk123/SpiecEasi/issues/90), are you planning on publishing NetCoMi on CRAN/Bioconductor? Conda would also b…
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Hi Benjamin,
Thanks for all your work on dada2 and this page.
I’ve come across something in a recent library that I havent been able to figure out from a few days exploring possibilities and trawl…