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I am plotting a heatmap from a matrix of 8 rows and 2284 columns. The columns are grouped manually by 8 clusters. My intention is to add a gap between clusters as follows:
```{r}
cell_annot
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### Name of the tool
umi-tools
### Tool homepage
https://github.com/CGATOxford/UMI-tools
### Tool description
A tool for dealing with UMIs
### Tool output
whitelist
```
# UMI-tools version: 1…
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I read in Lior's preprint X thread that splitcode would work well with long-reads, do you have any example of how to implement it for this case?
I would like to test it to demultiplex single-cell lo…
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Hi Dr. Wang,
I have just read your paper "A mouse model with high clonal barcode diversity for joint lineage,
transcriptomic, and epigenomic profifiling in single cells" and fairly interested in the…
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Project UUID: f81efc03-9f56-4354-aabb-6ce819c3d414
Project Title: Cell hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics
Project Short Name: Multip…
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I have a paired-end single-cell RNA-seq dataset. R1 consists of all the reads, and R2 of the barcodes necessary to identify which cell a read belongs to. If I now only trim R1 to keep high-quality rea…
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Hello,
I used TRUST4 to align VDJ region of gamma delta T cells. I used the cellranger output sample_alignments.bam file to align unassigned sequences
```
#the code Tianna ran
run-trust4 -b /…
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Hi there,
Congrats on a great work, and thanks for developing this package.
I am attempting to pull TCR-seq data for T cells that also have the GEX/CITE-seq Seurat objects.
**QUESTION 1.** …
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I'm trying to use dropEst to process SPLiT-seq data, specifically using SRR6750042 from GSE110823 as a test run. Note that I am able to successfully run dropEst on the example data (inDrop) to complet…
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I've been using STARsolo for single-cell RNA-seq analysis, and I have a question about how it handles SMART-seq data. I understand that SMART-seq does not employ UMIs or cell barcodes, which are typic…