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In the [usage page](https://github.com/WGLab/DeepMod/blob/master/docs/Usage.md) it is stated that FAST5 must be basecalled and events data must be available in them. However, it seems that the latest …
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Hi,
I try to run unicycler for a hybrid assembly and when I arrive to the step "Aligning read" after the step "SPAdes assemblies", the run is stuck to 100% :
Aligning reads (2018-09-06 18:17:33…
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### Ask away!
Hello, I would like to ask the following question:
We use **a novel 3' library preparation method** different from 10X (the main difference is that the barcode length of 10X v3 3prim…
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Bug report by @irosenboom
It was reported for 2x150bp Novaseq reads that bwa mem performed alignments with extensive softclipping.
For example, some alignments were of 18bp with 82 bp simply so…
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Hi, I user Shasta v0.11.1 to assemble a R10.4 dataset, the N50 of the assembly is only 3.4M, is this right? The dataset was download from s3://ont-open-data/giab_2023.05/analysis/hg002/sup/PAO83395.pa…
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> Medaka is a Research Release.
>
> Research releases are provided as technology demonstrators to provide early
> access to features or stimulate Community development of tools. Support for
> this…
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Hi Ben,
I have been using DADA2 on illumina sequenced COI data for the last year and it has become the backbone of my bioinformatics pipeline. We now have a PhD student in our group developing meta…
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See a great example here: [https://github.com/isugifNF/blast/blob/master/main.nf](https://github.com/isugifNF/blast/blob/master/main.nf).
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Hi, firstly thank you for creating the nabseq_nf pipeline.
I've been running the nabseq pipeline on Seqera/Tower and noticed that the biggest bottleneck is the `subset_aligned_reads ` step, which f…
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The genome size of the species I'm assembling is 2.6Gb, and the ONT data comes from dorado sup v5.0.0 (R10).
When I used 167GB data (>Q7, >10Kb) with "Nanopore-UL-May2022.conf", I got an assembly o…