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Hello
Let me explain briefly my issue I hope we can find a solution
I sequenced short tandem repeats (VNTR) using the Nanopore Native Barcoding Kit, (multiplexing)
each library contains only s…
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This behaviour is present on firmware version `8.2.0`. It could be that this is fixed by a higher version of the firmware (I'm guessing that c38114648a5c57cd7de2f3135e0fc05c7f82df98 could maybe help, …
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Hi,
1. Could SV2 take a region of SV to genotype like chr21 as input file? I want to test the pipeline on my real data. I get the a VCF of SV from Manta, only including chr21 SVs. Using the followi…
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@FA-Bubba & @GrahamDLL
Let's discuss 2.0 development here.
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Hello
Sorry to ask a non-issue related question but I wanted to ask a general question with using canu for plant genome assembly using nanopore data.
We have a~100X coverage for our plant using na…
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Some contigs are mostly simple repeats. The seeding and filling algorithm used in BLASR has trouble to align the reads to those contigs efficiently. It might make sense to detect shorter contig with …
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Hi Gene,
As I mentioned in [a previous issue](https://github.com/thegenemyers/DASCRUBBER/issues/6), I wrote [this wrapper script](https://github.com/rrwick/DASCRUBBER-wrapper) to use your tools for…
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Greetings,
I am interested in trying the LTR_retriever strategy to validate hits I obtained from an LTRharvest dataset. I downloaded the files associated with LTR_retriever and modified the "paths"…
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Hello,
I have compiled this and it is running but I get a "kill" result on Step 1 and the log last lines reads "TRF output lines processed 32481800". The mail at the bu web site is inop.
When…
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In many assemblies we are finding that assembly graph contiguity is not much higher than that of contigs extracted from it. This is true not just for miniasm but also other assemblers.
Why might th…